Rehmannia glutinosa root and Saururus chinensis plant have been widely used in natural traditional medicines. This study was conducted to investigate the effect of the content of the main components of R. glutinosa root and S. chinensis plant by extraction method. The results of comparative analysis of extraction yield, total flavonoid, and polyphenol content by extraction method indicated that extraction yield had the best performance with hot water extraction for R. glutinosa and ultrasound-assisted extraction for S. chinensis. The total flavonoid and polyphenol content had the best performance with maceration extraction for R. glutinosa and ultrasound-assisted extraction for S. chinensis. ABTS and DPPH activity was excellent with maceration extraction for both R. glutinosa and S. chinensis. The analysis of the main components showed that maceration extraction was most effective for both S. chinensis and R. glutinosa. Specifically, maceration extraction of R. glutinosa yielded 1.5 times more than conventional ultrasound-assisted extraction.

Introduction
Materials and methods
    1. Plant material
    2. Extraction methods
    3. Analyzing total polyphenol and flavonoid content
    4. Determination of antioxidant activity (ABTS andDPPH)
    5. Determination of main components in R. glutinosaand S. chinensis
    6. Statistical Analysis
Results and discussion
    1. Analysis of extraction yield and antioxidantsubstance according to extraction method
    2. Analysis of antioxidant activity according toextraction method
    3. Analysis of main components of R. glutinosa andS. chinensis according to extraction method
References

• Jong Hyuk Kim(Ph.D. student, Department of Applied Life Science, Gyeongsang National University, Jinju 52828, Korea)
• Ju Hyun Ha(The master´s course student, Department of Agronomy, Gyeongsang National University, Jinju 52828, Korea)
• Il Rae Rho(The master´s course student, Department of Agronomy, Gyeongsang National University, Jinju 52828, Korea, Professor, Institude of Agriculture Life Sciences, Gyeongsang National University, Jinju 52828, Korea) Corresponding author