This study examined the spatial morphological patterns of forest habitats and the characteristics of roadkill occurrences in the forests of Mungyeong, Yecheon, Yeongju, Andong, and Bonghwa in Gyeongsangbuk-do. It involved building a resistance map between habitats and analyzing connectivity based on the least-cost distance. The analysis of the distance between the forest habitat Cores derived from MSPA and roadkill points showed that roadkill occurrences were concentrated approximately 74.11 m away from the Cores, with most roadkills happening within 360 m from the habitats. The connectivity analysis between core habitats larger than 1 km2 revealed 141 core habitats and 242 least-cost paths between them. The corridor distance value was found to be highest in Mungyeong city, indicating an urgent need for strategies to enhance habitat connectivity there. This research is expected to serve as foundational data for developing strategies to enhance ecosystem connectivity and restore habitats, by analyzing ecosystem connectivity and roadkill issues due to habitat fragmentation.