To understand the detrimental effects of triclosan on Java medaka (Oryzias javanicus) embryos, fertilized embryos were exposed to different concentrations (1, 10, 50, 100, 200, 400, 600, 800, and 1,000 μg l-1) of triclosan until hatching. Then, we examined the survival rate and developmental parameters as well as alterations in antioxidant constituents and DNA damage markers. The results showed dose-dependent mortality, hatching delays, and developmental abnormalities in the embryos. Additionally, there were significant increases in oxidative stress parameters and antioxidant responses, along with elevated DNA damage. These findings suggest that sublethal concentrations of triclosan induce toxic effects through oxidative stress on Java medaka embryos, as evidenced by changes in in vivo parameters and biochemical constituents.

Introduction
Materials and Methods
    1. Java medaka
    2. Exposure
    3. Measurement of biochemical parameters
    4. DNA stability
    5. Statistical analysis
Results and Discussion
Acknowledgment
Conflict of Interest
References

• Seong Duk Do(Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, Korea)
• Jae-Sung Rhee(Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, Korea, Research Institute of Basic Sciences, Incheon National University, Incheon 22012, Korea, Yellow Sea Research Institute, Incheon 22012, Korea) Corresponding author