This study sought to confirm the inhibitory ability of Clostridioides difficile KCTC 5009 strain using the bacteriocin-producing Lactococcus lactis CJNU 3001 strain. For this purpose, bacterial culture and partially purified bacteriocin verified the antibacterial activity against C. difficile strain. As a result, both samples showed strong antibacterial activity against the target strain. Co-culture tests were performed to more closely analyze the competition between the two strains. As a result, a similar inhibitory effect was confirmed in RCM broth and CMM (colon model medium) broth. In particular, inhibition of C. difficile KCTC 5009 strain was not observed until the exponential phase of the growth of L. lactis CJNU 3001 strain, because bacteriocins were produced during that period. It was inferred that it actively suppressed the growth of the target strain after that. Therefore, as it was proven that the bacteriocin-producing L. lactis CJNU 3001 strain can effectively control C. difficile strain in co-cultures, it was judged that it could be an alternative for CDI (Clostridioides difficile infection) treatment.

서 론
재료 및 방법
    사용 균주
    C. difficile 균주에 대한 L. lactis 균주의 항균 활성
    박테리오신 부분 정제 및 항균작용 양상
    L. lactis 균주와 C. difficile 균주의 경쟁
    통계처리
결과 및 고찰
    C. difficile 균주에 대한 L. lactis 균주의 항균 활성
    박테리오신 부분 정제 및 항균작용 양상
    L. lactis 균주와 C. difficile 균주의 경쟁
요 약
감사의 글

• 이호진(한국교통대학교 생명공학전공) | Ho-Jin Lee (Department of Biotechnology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea)
• 홍예빈(한국교통대학교 생명공학전공) | Ye-bin Hong (Department of Biotechnology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea)
• 이재혁(한국교통대학교 생명공학전공) | Jae-Hyuk Lee (Department of Biotechnology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea)
• 문기성(한국교통대학교 생명공학전공) | Gi-Seong Moon (Department of Biotechnology, Korea National University of Transportation, Jeungpyeong 27909, Republic of Korea) Corresponding author