Drought is one of the environmental factors inhibiting plant productivity and growth, leading to oxidative damage. This study aims to identify the role of sodium hydrosulfide (NaHS) as a hydrogen sulfide (H2S) donor in drought stress tolerance in Brassica napus. Drought-induced stress symptoms appeared eight days after treatment, showing wilted leaves and a significant reduction of leaf water potential. Drought-induced increase of lipid peroxidation was significantly reduced by NaHS application. NaHS-treated plants mitigated stress symptoms under drought conditions by reducing hydrogen peroxide (H2O2) content, confirmed with H2O2 localization in situ. Furthermore, NaHS promotes photosynthetic activity by maintaining chlorophyll and carotenoid content, thereby supporting plant growth under drought conditions. Pyrroline-5-carboxylate and proline contents were significantly increased by drought but further enhanced by NaHS treatment, indicating the important roles of proline accumulation in drought stress tolerance. In conclusion, this study provides valuable insight into the roles of NaHS in alleviating drought stress by reducing oxidative stress and promoting proline accumulation. Therefore, NaHS may serve as an effective strategy to enhance crop production under drought-stress conditions.

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Plant materials and treatments
    2. Determination of leaf water potential and lipidperoxidation
    3. Determination of H2O2 content and H2O2 visualization
    4. Determination of chlorophyll and carotenoid content
    5. Determination of proline and P5C content
Ⅲ. RESULTS
    1. Physiological symptoms of plants
    2. Leaf water potential and MDA content
    3. H2O2 visualization and H2O2 content
    4. Chlorophyll and carotenoid content
    5. Proline and P5C content
Ⅳ. DISCUSSION
Ⅴ. ACKNOWLEDGEMENTS
Ⅵ. REFERENCES

• Septi Anita Sari(Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea)
• Muchamad Muchlas(Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea)
• Bok-Rye Lee(Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea)
• Md Al Mamun(Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea)
• Tae-Hwan Kim(Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea) Corresponding author