The Yeongsan River is a prominent inland waterway, alongside the Han River, Nakdong River, and Geum River in South Korea. Numerous bacterial strains were isolated from the Yeongsan River basin for a comprehensive investigation into indigenous prokaryotic species conducted between 2020 and 2023. These bacterial strains were identified using 16S rRNA gene sequencing, wherein 45 bacterial strains shared >98.7% sequence similarities with bacterial species not recorded in Korea thus far. Therefore, this study aimed to catalogue aforementioned unrecorded species and characterize them contingent upon their Gram nature, colony and cell morphologies, biochemical properties, and phylogenetic positions. These bacterial species were determined to be phylogenetically diverse. They were categorized into nine classes, 18 orders, and 25 families. These previously unrecorded species were classified into the following genera and classes: Chitinophaga (class Chitinophagia); Flavobacterium (class Flavobacteriia); Rhodopseudomonas, Gemmobacter, Paracoccus, Azospirillum, Sphingomonas, Novosphingobium, Sphingorhabdus, and Erythrobacter (class Alphaproteobacteria); Bordetella, Pararobbsia, Polynucleobacter, Rhodoferax, Aquabacterium, Malikia, Comamonas, Ideonella, Paucibacter, Undibacterium, Cupriavidus, and Thauera (class Betaproteobacteria); Pectobacterium, Arenimonas, Lysobacter, and Luteimonas (class Gammaproteobacteria); Luteolibacter (class Verrucomicrobiia); Mycolicibacterium, Angustibacter, Ornithinibacter, Janibacter, Schumannella, Aurantimicrobium, Luedemannella, Nocardioides, and Propionicimonas (class Actinomycetes); Geothrix (class Holophagae); and Lactococcus (class Bacilli).

1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
    3.1. Description of Chitinophaga silvisoli20YS10WR-12
    3.2. Description of Flavobacterium lacunaeR20SS-10
    3.3. Description of Flavobacteriumsaliperosum R20SS-19
    3.4. Description of Flavobacteriumurocaniciphilum 21YS07S-S28
    3.5. Description of Rhodopseudomonasfaecalis 01TPW-2-14
    3.6. Description of Gemmobacter tilapiae20CGW-82
    3.7. Description of Paracoccus luteus20CGW-69
    3.8. Description of Azospirillum agricolaYGJ-S-30
    3.9. Description of Sphingomonas mesophilaTM2-4
    3.10. Description of Novosphingobium piscinae20YS06W0N-35
    3.11. Description of Sphingomonas laterariae20YS10WR-09
    3.12. Description of Sphingomonas lacunae21YS08-0M08
    3.13. Description of Sphingorhabdusplanktonica 21YS05S-0R27
    3.14. Description of Erythrobacter sanguineus21YS03W-49
    3.15. Description of Bordetella hinzii20YS06W0N-38
    3.16. Description of Pararobbsia alpina SJ2-62
    3.17. Description of Polynucleobacteryangtzensis 21YS05W-0N23
    3.18. Description of Rhodoferax bucti T10
    3.19. Description of Aquabacterium pictum01MPW-2-28
    3.20. Description of Malikia spinosa01TPW-2-35
    3.21. Description of Comamonas kerstersiiYGS-S-32
    3.22. Description of Ideonella paludis SN-1
    3.23. Description of Paucibacter toxinivorans21YS02S-57
    3.24. Description of Undibacterium amnicola20Ga-4
    3.25. Description of Cupriavidus agavae21YS01W-91
    3.26. Description of Thauera terpenica21YS16S-07
    3.27. Description of Pectobacteriumpunjabense R20SW-21
    3.28. Description of Arenimonas malthaeYGS-S-16
    3.29. Description of Lysobacter xinjiangensis20YS10WR-01
    3.30. Description of Luteimonasnotoginsengisoli 21YS07S-S12
    3.31. Description of Luteolibacter luojiensis21YS10W-ISP05
    3.32. Description of Mycolicibacterium vaccae20DYS-68
    3.33. Description of Angustibacter luteusSN-2
    3.34. Description of Ornithinibacter aureus21YS16S-51
    3.35. Description of Janibacter cremeusNM40
    3.36. Description of Aurantimicrobiumminutum 21YS14W-18
    3.37. Description of Luedemannella flava20DYS-41
    3.38. Description of Nocardioidesiriomotensis 20SRW-23
    3.39. Description of Nocardioidesszechwanensis TM2-1
    3.40. Description of Schumannella luteola20YS09W-41
    3.41. Description of Propionicimonaspaludicola 20DYW-34
    3.42. Description of Nocardioides aquaticus21YS13S-68
    3.43. Description of Nocardioides simplex21YS07S-S44
    3.44. Description of Geothrix fermentans21YS21S-6
    3.45. Description of Lactococcus hircilactisR20SW-11
CRediT authorship contribution statement
Declaration of Competing Interest
ACKNOWLEDGEMENTS
REFERENCES

• Sanghwa Park(Bio-resources Bank Division, Using Technology Development Department, NNIBR, Sangju 37242, Republic of Korea) Corresponding author
• Seok Won Jang(Protist Research Division, Microbial Resources Research Department, NNIBR, Sangju 37242, Republic of Korea)
• Soo-Yeong Lee(Prokaryote Research Division, Biological Resources Research Department, NNIBR, Sangju 37242, Republic of Korea)
• Ja Young Cho(Prokaryote Research Division, Biological Resources Research Department, NNIBR, Sangju 37242, Republic of Korea)
• Jung Hye Eom(Prokaryote Research Division, Biological Resources Research Department, NNIBR, Sangju 37242, Republic of Korea)
• Eui Jin Kim(Bio-resources Research Division, Using Technology Development Department, NNIBR, Sangju 37242, Republic of Korea)