논문 상세보기
pp.303-310
This study investigated immunomodulatory and antimetastatic properties of polysaccharides extracted from hallabong (Citrus hybrid) peel, focusing on their effects on macrophage activation. Crude polysaccharides (HPWP) were obtained through hot water extraction and ethanol precipitation from dried hallabong peel powder. Macrophages isolated from mouse peritoneum were used to evaluate cytotoxicity of HPWP against normal cells and Colon 26-M3.1 carcinoma cells. Our findings revealed no cytotoxic effects of HPWP on either normal cells or Colon 26-M3.1 carcinoma cells. Immunoactivity was assessed by measuring cytokine production, specifically IL-6 and IL-12, using ELISA. Results indicated a significant, concentration-dependent increases of cytokine levels. Tumoricidal activity of NK cells was also evaluated, demonstrating enhanced tumor cell killing induced by HPWP, in a concentration-dependent manner. This increased activity was due to HPWP’s potent antimetastatic effects, as observed in a mouse model of Colon 26-M3.1 carcinoma. In comparison with a the negative control (NC, 100%), HPWP at concentrations of 10, 100, and 1000 μg/mouse showed antimetastatic effects of 49.4%, 61.3%, and 74.3%, respectively. These effects were attributed to only NK cell activation. These findings suggest that HPWP has potential as a functional ingredient in food products aimed at enhancing immune response and inhibiting cancer metastasis.
서 론
재료 및 방법
1. 한라봉 과피로부터 다당 추출
2. 일반 분석
3. 구성당 분석
4. 실험동물
5. 대식세포 및 Colon26-M3.1 암세포에 대한 독성 측정
6. Cytokine 생산능 측정
7. NK cell 측정
8. 항전이 효과 측정
9. NK cell의 기능이 억제된 마우스에서의 항전이 효과측정
10. 통계처리
결과 및 고찰
1. 한라봉 과피 유래 다당의 일반성분
2. 한라봉 과피 유래 다당의 정상세포 및 암세포 독성
3. 한라봉 과피 유래 다당의 cytokine 생산능
4. 한라봉 과피 유래 다당의 NK cell 활성능
5. 한라봉 과피 유래 다당의 항전이 효과
6. NK cell이 억제된 마우스에서 한라봉 과피 유래 다당의 항전이 효과
요약 및 결론
