This research introduces a novel probabilistic approach to consider the effects of uncertainty parameters during the design and construction process, providing a fresh perspective on the evaluation of the structural performance of reinforced concrete structures. The study, which categorized various random design and construction process variables into three groups, selected a two-story reinforced concrete frame as a prototype and evaluated it using a nonlinear analytical model. The effects of the uncertainty propagations to seismic responses of the prototype RC frame were probabilistically evaluated using non-linear dynamic analyses based on the Monte-Carlo simulation sampling with the Latin hypercube method. The derivation of seismic fragility curves of the RC frame from the probabilistic distributions as the results of uncertainty-propagation and the verification of whether the RC frame can meet the seismic performance objective from a probabilistic point of view represent a novel and significant contribution to the field of structural engineering.