Background: Because oxidative stress can induce decreased quality of caprine semen during the storage, there has been limitation for the use of stored semen in the assisted reproductive technologies. The present study, therefore, assesses the potential of Annona muricata (A. muricata ) to reduce semen storage associateddamages. Methods: Semen was collected by electro-ejaculation from ten bucks, and extended with Tris-egg yolk (TEY) supplemented with A. muricata leaf aqueous extract (SAE) at 20 (SAE20), 40 (SAE40), and 80 (SAE80) μg/mL. Sperm variables including motility, motion characteristics, viability, membrane functionality, and DNA integrity were assessed at different storage periods (6, 24, 48, and 72 hr). In addition, oxidative stress indicators in the extender supplemted with SAE were also assessed for each group. Results: By adding SAE at 80 μg/mL in TEY, the storage of goat buck semen was improved, resulting in reduced loss of sperm motility, viability, DNA fragmentation, and membrane integrity during chilled storage at 4℃ for up to 72 hr. In addition, enrichment of TEY extender with SAE significantly (p < 0.05) reduced malondialdehyde, an indicator of oxidative stress, compared to the negative control. Conclusions: Supplementation of SAE in TEY extender can reduce buck spermatozoa liquid storage associated damages due to oxidative stress.

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
    Study site
    Annona muricata (Soursop) leaves harvesting andpreparation of aqueous extract
    Animals and semen collection
    Extender preparation and semen processing
    Assessment of sperm viability
    Assessment of sperm functional membrane integrity
    Assessment of sperm DNA integrity
    Assessment of sperm motility
    Assesment of total peroxidases
    Assesment of lipid peroxidation
    Assessment of superoxide dismutase activity
    Assessment of catalase activity
    Statistical analysis
RESULTS
    Effects of SAE on buck’s sperm total motility,progressive motility, and local motility
    Simple interactions of time and doses of SAE on buck’s sperm total motility, progressive motility, and localmotility
    Effects of SAE on buck’s sperm motion characteristics
    Simple interactions of time and doses of SAE on buck’s sperm motion characteristics
    Effects of SAE on buck’s sperm viability, plasmamembrane and DNA integrity
    Simple interactions of time and doses of SAE on buck’s sperm viability, plasma membrane and DNA integrity
    Effects of incorporating SAE in TEY extender on lipidperoxidation biomarkers content in buck’s semen
    Simple interactions of time and doses of SAE in TEYextender on oxidative stress biomarkers content inbuck’s semen
DISCUSSION
CONCLUSION
REFERENCES

• Mohamadou Adamou(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon, Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon) Corresponding author
• Dongmo Nguedia Arius Baulland(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon)
• Ngo Bahebeck Pierrette(Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon)
• Tchoffo Herve(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon)
• Chongsi Margaret Mary Momo(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon)
• Noudjio Kezeter Claude(Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon)
• Nnanga Germaine Estelle Mvondo(Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon)
• Ngwemetah Nathalie(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon)
• Adamu Yusufa Njeiri(College of Technology, University of Bamenda, Bamenda 39, Cameroon)
• Ngoula Ferdinand(Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon)