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In vitro Asymbiotic Germination of Immature Seed and Seedling Development of Wild White-egret Flower (Habenaria radiata (Thunb.) Spreng) in Korea KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/437994
pp.336-345
Habenaria radiata, commonly known as the Egret Flower, is a critically endangered terrestrial orchid facing rapid population decline due to overharvesting in Korea. Asymbiotic seed propagation offers a promising conservation method for this rare species. This study investigates the effects of different nutrient media on the germination and plantlet development of immature seeds of H. radiata, with the goal of establishing optimal propagation conditions. Among four media types, with the highest germination rate (75.6%) observed on OBTSG medium, followed by 1/2 MS, OMM, and OSGM. Protocorm development varied across media, with OBTSG and OMM supporting the most robust growth. A two-step culture method involving OBTSG for germination followed by OMM for plantlet development proved most effective, achieving an 84.4% survival rate. These findings highlight the importance of media composition and transitioning for successful propagation. The results provide a practical protocol for in vitro propagation, contributing to both in situ and ex situ conservation efforts aimed at preserving H. radiata and other endangered terrestrial orchids.
Materials and Methods
Collection and in-vitro establishment
Asymbiotic media effects on seed germination
Development and growth of protocorm
Statistical analysis
Results
Immature seed and stainability of seeds with TTC
Asymbiotic media effects on seed germination
Development and growth of protocorm
Discussion
Acknowledgments
References
