This study investigated the growth dynamics of Lilium miquelianum bulb scales cultured in four different media formulations: Full Murashige-Skoog (FMS), Half Murashige-Skoog (HMS), Anderson Vitamin (AV), and Knudson Vitamin (KV). Bulb scales were collected from native populations on Jeju Island, Korea, and cultured in vitro for 12 weeks. Growth parameters, including bulb area and scale count, were measured at 3-week intervals. Statistical analyses revealed significant differences in growth rates across media types and time points. FMS consistently demonstrated the highest growth rates and final bulb area and scale count, while KV exhibited the lowest performance. Heatmap analysis showed that FMS achieved the highest weekly growth rates in most time points, with HMS showing comparable performance during early growth stages. Individual sample growth rates varied within media, highlighting the influence of both external and internal factors on growth. Correlation analysis revealed a positive association between bulb size and scale quantity, with FMS exhibiting the strongest relationship. These findings emphasize the importance of appropriate media selection for optimal L. miquelianum propagation, with FMS being the most suitable for extended cultivation. This research provides valuable insights for improving propagation efficiency and conservation efforts of L. miquelianum.

Introduction
Materials and Methods
    In vitro Plant Materials
    Statistical Analysis
    Normalization and Relative Growth Calculation
    Visualization and Correlation Analysis andData integrity
Results and Discussion
    Growth Dynamics Across Media Over Time
    Variability in Growth Across Media and Samples
Conclusion
Acknowledgments
References

• Juyeong Seo(Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea)
• Yunqing Ji(Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea)
• Ji-Yun Kang(Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea)
• Ki-Byung Lim(Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea)
• Yun-Jae Ahn(Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea, Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Korea) Corresponding author