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Sulfite Lignin Enhances Seed Germination and Salt Stress Tolerance KCI 등재
pp.264-269
Humic acids (HA), with their irregular polymeric structures and largely existing in grassland, present challenges in quality control due to significant variations in biological activities depending on extraction sources. To address this, we explored industrial byproducts as potential alternatives mimicking HA-like bioactivities. This study evaluates sulfite lignin, a byproduct of the pulp industry, as an eco-friendly biostimulant for enhancing plant growth and stress tolerance. Sulfite lignin demonstrated HA-like bioactivities, promoting seed germination and salt stress tolerance in Arabidopsis thaliana. Germination assays revealed that sulfite lignin significantly improved radicle and cotyledon emergence, particularly at low concentrations (8.6 mg L⁻¹), outperforming HA and kraft lignin. Additionally, under salt stress conditions, sulfite lignin-treated plants exhibited healthier phenotypes and maintained higher chlorophyll content compared to control treatments, similar to HA and kraft lignin. The findings highlight sulfite lignin as a promising, sustainable, and cost-effective biofertilizer, effectively replicating HA's biological functions while leveraging industrial byproducts.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Plant materials and growth conditions
2. Germination assay on HA, kraft lignin and sulfitelignin-containing medium
3. Salt stress tolerance assay
4. Total chlorophyll content
5. Statistical analysis
Ⅲ. RESULTS AND DISCUSSION
1. Sulfite lignin promotes seed germination
2. Sulfite lignin enhances salt stress tolerance
Ⅳ. CONCLUSION
Ⅴ. ACKNOWLEDGMENTS
Ⅵ. REFERENCES
