Ganoderma lucidum, a fungus of significant scientific interest that encompasses a diverse array of bioactive compounds, has been extensively investigated for its potential health-promoting and disease-preventing properties. Ganodermanontriol, a triterpenoid, is the principal active component contributing to its biological activities. This study aimed to explore the anti-inflammatory properties of ganodermanontriol and to evaluate its potential as a functional ingredient. The expression of inflammatory mediators, including tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS), and nitric oxide (NO) production was significantly inhibited in ganodermanontriol (1.25–5 μg/mL)-treated compared to that of untreated lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Additionally, the phosphorylation of mitogen-activated protein kinases (MAPK), specifically p38 and c-Jun N-terminal kinase (JNK), was markedly inhibited in ganodermanontriol (3–5 μg/mL)-treated cells. These findings indicate that ganodermanontriol possesses significant potential as both a prophylactic and therapeutic agent for immune-related disorders, owing to its ability to modulate inflammatory responses.

ABSTRACT
서 론
재료 및 방법
    세포배양
    세포 생존율 평가
    RAW 264.7 세포주에 대한 산화질소 (NO) 생성 억제능 평가
    Total RNA 추출 및 실시간 정량 PCR
    단백질 추출 및 western blot
    통계 처리
결과 및 고찰
    가노더마논트리올이 세포 생존율 및 산화질소 (NO) 생성 억제에 미치는 영향
    가노더마논트리올이 염증 매개 인자 발현에 미치는 영향
    가노더마논트리올이 iNOS 발현 및 MAPK 단백질 인산화에 미치는 영향
적 요
감사의 글
REFERENCES

• 박채훤(건국대학교) | Che-Hwon Park (Department of Medicinal Biosciences, College of Biomedical and Health Science, Konkuk University, Chungju 27478, Korea)
• 이성철(건국대학교) | Seong-Cheol Lee (Department of Medicinal Biosciences, College of Biomedical and Health Science, Konkuk University, Chungju 27478, Korea)
• 박영진(건국대학교) | Young-Jin Park (Department of Medicinal Biosciences, College of Biomedical and Health Science, Konkuk University, Chungju 27478, Korea) Corresponding author