Background: The diaphragm release technique, commonly used in COPD patients, improves diaphragmatic mobility and inspiratory capacity. Diaphragmatic breathing, with slow deep breaths, supports autonomic regulation and benefits the brain, cardiovascular, and respiratory systems. However, research on their combined effects on respiratory function remains limited. Objectives: To investigate the effects of manual diaphragm release on respiratory functions, specifically focusing on forced vital capacity (FVC), forced expiratory volume in one second (FEV1), diaphragmatic mobility, and chest expansion. Design: A randomized controlled trial. Methods: The study involved 26 university students, who were randomly assigned to either the experimental group (Manual diaphragm release + Diaphragmatic breathing, MDR) or the control group (Diaphragmatic breathing, DB). Measurements taken before and after the immediate intervention included diaphragmatic mobility, chest expansion, FVC, and FEV1. Results: In the experimental group, significant differences were observed in diaphragmatic mobility, FVC, and FEV1 between the pre- and post-intervention measurements, while the control group had no significant changes. The experimental group showed significant improvements in diaphragmatic mobility and FVC compared to the control group. Conclusion: MDR significantly enhance diaphragmatic mobility and FVC in university students compared to DB. These findings suggest that MDR is an effective non-invasive intervention for improving specific respiratory functions.