논문 상세보기
pp.1637-1647
The cosmetics industry expects skin improvement and anti-aging effects by using natural products and natural extracts. In particular, herbal extracts are ingredients with traditionally proven pharmacological efficacy, and are in the spotlight as effective ingredients for maintaining skin health and improving skin troubles. In this study, the antioxidant, anti-inflammation, and wrinkle improvement effects were evaluated by using herbal ingredients such as Dioscorea opposita, Angelica dahurica, Rehmannia glutinosa, Panax ginseng and Ophiopogon japonicus. The antioxidant activity of the herbal complex was confirmed by measuring DPPH and ABTS+ radical scavenging activity, and after a cytotoxicity test using the MTT assay, it showed a concentration-dependent inhibition effect on NO production by LPS stimulation in RAW 264.7 cells. In addition, in the analysis of MMP-1, MMP-2 and procollagen gene expression induced by H2O2 in HDF cells, the expression of MMP-1 was suppressed in a dose-dependent manner by the herbal complexes. This is expected to make a significant contribution to wrinkle improvement and skin elasticity enhancement. These results suggest that herbal complexes can be used as promising cosmetic materials for skin improvement and anti-aging.
1. 서 론
2. 실 험
2.1. 시료의 추출
2.2. 재료 및 기기
2.3. DPPH 라디칼 소거 활성 측정
2.4. ABTS+ 라디칼 소거 활성 측정
2.5. 세포 배양
2.6. MTT 분석을 통한 세포 독성 측정
2.7. Nitric Oxide (NO) 저해능 측정
2.8. 유전자 발현 분석을 위한 RNA 분리 및RT-PCR
2.9. 통계처리
3. 결과 및 고찰
3.1. DPPH radical 소거능 측정
3.2. ABTS+ radical 소거능 측정
3.3. MTT assay 측정 결과
3.5. 한방 복합물의 Nitric oxide(NO) 저해능
3.6. HDF 세포에서 한방 복합물 처리에 의한유전자 발현 변화 분석
4. 결 론
감사의 글
References
