본 연구는 기능성 화장품 소재 개발을 목표로 효모 유래 MPC의 세포 생리활성을 조사하였 다. 피부 세포주에 처리된 Cu와 Zn 이온 모두 세포 독성이 확인되었지만, 정제된 MPC는 결합된 금속 이온의 세포 독성을 획기적으로 제거하였다. 게다가 특정 농도의 MPC는 대조군과 비교하여 세포 생존 율을 오히려 약 20% 증가시켰다. MPC 중 효모 펩타이드-Cu(YP-Cu)는 UVB 자극으로 유도되는 세포 내 활성산소의 양을 약 30% 정도 유의하게 감소시켰지만, YP-Zn은 영향을 미치지 못했다. 또한, YP-Cu 처리는 피부 세포에서 콜라겐 유전자의 발현량을 2배 증가시켰고, 프로콜라겐 분비량은 1.7배 증 가시켰으며, UVB 자극에 의한 콜라겐 유전자의 발현 저해에도 효과적으로 대응했다. 결론적으로, 유리 금속 이온 자체는 세포독성 효과로 인해 화장품 소재에 적합하지 않지만, 정제된 MPC, 특히 YP-Cu는 이러한 금속 이온의 독성을 효과적으로 상쇄하고 세포 생존율을 향상시킬 뿐만 아니라, UVB 자극에 따 른 유해 효과를 완화하기 때문에 잠재적 기능성 화장품 소재로 사용될 수 있다.

The objective of this study was to investigate the cellular bioactivity of yeast-derived Metal Peptide Complexes (MPCs) for the development of functional cosmetic ingredients. Both copper (Cu) and zinc (Zn) ions demonstrated cytotoxicity in skin cell lines; however, purified MPCs effectively neutralized the cytotoxicity of these metal ions. Remarkably, certain concentrations of MPCs increased cell viability by approximately 20% compared to the control group. Among the MPCs, yeast peptide-Cu (YP-Cu) significantly reduced intracellular reactive oxygen species (ROS) levels induced by UVB stimulation by approximately 30% compared to the control group, whereas YP-Zn had no effect. Additionally, YP-Cu treatment increased collagen gene expression in skin cells by 2-fold and procollagen secretion by 1.7-fold. It also effectively mitigated the inhibition of collagen gene expression caused by UVB stimulation. In conclusion, while free metal ions themselves are unsuitable for cosmetic ingredients due to their cytotoxic effects, purified MPCs, particularly YP-Cu, can effectively counteract the toxicity of these metal ions, enhance cell viability, and mitigate the harmful effects of UVB stimulation, thereby representing potential functional cosmetic ingredients.

요 약
Abstract
1. Introduction
2. Experiments
    2.1. Materials
    2.2. Preparation of Yeast peptide
    2.3. Cell Viability Assay
    2.4. ROS Measurement
    2.5. RNA extraction and RT-qPCR
    2.6. ELISA of Procollagen-I (PC-I)
    2.7. Statistical Analysis
3. Results and Discussion
    3.1. Investigation of Cytotoxicity of MetalIons and Yeast Lysate (YL)
    3.2. Investigation of Cytotoxicity of PurifiedMetal Peptide Complexes (MPCs)
    3.3. Investigation of Intracellular ReactiveOxygen Species (ROS) Generation byMPCs Treatment
    3.4. Investigation of Collagen Expressionand Secretion by MPCs Treatment
4. Conclusion
Acknowledgement
References

• Che-Yeon Hong(Department of Biotechnology, Korea National University of Transportation, Student)
• Da-Won Jung(Department of Biotechnology, Korea National University of Transportation, Student)
• Yu-Bin Lee(Department of Biotechnology, Korea National University of Transportation, Student)
• Nadia Begum(Department of Biotechnology, Korea National University of Transportation, Student)
• Seong-Min Lee(Macrocare Co., Ltd, 32, Gakri 1-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungbuk)
• Moo-Sung Kim(Macrocare Co., Ltd, 32, Gakri 1-gil, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungbuk)
• Gi-Seong Moon(Department of Biotechnology, Korea National University of Transportation, Professor) Corresponding author
• Hyang-Yeol Lee(Department of Biotechnology, Korea National University of Transportation, Professor) Corresponding author
• Jun-Sub Kim(Department of Biotechnology, Korea National University of Transportation, Professor) Corresponding author