Sweetpotato (Ipomoea batatas L.) is a globally important food crop susceptible to root-knot nematode (Meloidogyne incognita) infection, which causes significant crop losses. Previous transcriptome and proteome analyses have identified several genes that show differential expression patterns in susceptible and resistant cultivars in response to root-knot nematode (RKN) infection. Among the genes responsive to this process, sporamin (SPO) genes were identified as the most abundant in the transcriptome analysis, and additional changes in the expression of SPO genes that showed specific responses to RKN infection were identified during nematode infection. In this study, we used SPO genes used in previous studies to identify expression patterns under various abiotic stress conditions. The expression of four SPO genes in sweetpotato leaves was investigated under conditions of drought, high salt, and stress-related chemicals treatment. The expression of G13675/TU22356, G34367/TU56356, and G34382/TU56395 increased similarly under dehydration and chemicals treatment conditions. On the other hand, G34367/TU56358 was constantly expressed during the stress treated conditions. This study is expected to be used as basic data on how the gene for the major protein SPO of sweetpotato responds to various abiotic stress conditions as well as nematode infection.