In this paper, the uniaxial tensile mechanical characteristics after synchronous biaxial pre-tension analysis are investigated on a ETFE film crown model. After the biaxial pre-stretching is completed, the rectangular specimens are cut along the MD and TD directions of the biaxially pre-stretched ETFE film to conduct the uniaxial stretching test. The uniaxial tensile mechanical properties of the ETFE film after biaxial pre-stretching are investigated, the specific uniaxial tensile mechanical property parameters of the biaxially pre-stretched film are determined, and the influence of biaxial pre-stretching on the uniaxial tensile mechanical properties of the ETFE film is analyzed.