논문 상세보기
Isolation and identification of organic compounds-degrading bacterium, Bacillus atrophaeus YBK-W4, for the treatment of organic wastes KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/443198
pp.51-57
In this study, a bacterial strain exhibiting high degradation activity toward organic wastes was isolated and taxonomically identified. Five strains with excellent hydrolytic activity toward starch, lipids, and proteins were isolated from slaughter waste processors. Among the five strains, YBK-W4, which showed the highest amylase, protease, and lipase activities, was selected. Through 16S rRNA sequencing, YBK-W4 was identified as Bacillus atrophaeus (B. atrophaeus) and designated B. atrophaeus YBK-W4. The optimal temperature and pH for enzyme activity of B. atrophaeus YBK-W4 were 37~42°C and pH 7, respectively. The bacterium was grown on Luria-Bertani (LB) agar containing 12% NaCl, where enzyme activity was 11.4~29.7% of that observed in LB agar without NaCl. These results suggest that B. atrophaeus YBK-W4 is a useful bacterial agent for the decomposition of organic waste, such as food and slaughterhouse waste.
서 론
재료 및 방법
균주분리
유기물 분해능 검색
생육조건별 유기물 분해효소 활성
16S rRNA 염기서열 분석 및 동정
결 과
유기물 분해능 검색
생육조건별 유기물 분해효소 활성
16S rRNA 염기서열 분석 및 동정
고 찰
REFERENCES
