Baicalin is known to exhibit neuroprotective effects during brain injury through its antioxidant and anti-inflammatory functions. Moreover, γ-enolase is specifically expressed in nerve cells and exhibits neuroprotective properties. In this study, we investigated whether baicalin regulates γ-enolase expression in an moddle cerebral artery occulsion (MCAO)-induced brain injury model. Adult male rats were intraperitoneally injected with baicalin (100 mg/kg) or phosphate-buffered saline (PBS) immediately after right MCAO surgery. Neurological behavior tests were performed 24 hours after surgery and brain water content was evaluated. Right cortical tissue was collected. Western blot analysis were conducted to elucidate γ-enolase expression in MCAO animals treated with baicalin. In addition, γ-enolase expression was analyzed using immunofluorescence staining. MCAO animals administered PBS displayed severe behavioral impairments and edema, whereas baicalin administration alleviated these disorders, demonstrating the protective effects of baicalin against ischemic damage. Western blot analysis results showed that MCAO-induced damage decreased γ-enolase expression, and baicalin treatment mitigated this reduction. These findings were confirmed through immunofluorescence staining. Since γ-enolase is known to contribute to neuroprotective effects, these results suggest that baicalin alleviates neurobehavioral impairments in stroke animals and exerts neuroprotective effects by attenuating the decline in γ-enolase expression caused by brain injury. In conclusion, we demonstrated that baicalin regulates γ-enolase expression during cerebral ischemic damage.

Abstract
서 론
재료 및 방법
    실험 동물
    중간대뇌동맥폐쇄술
    코너 테스트(Corner test)
    그립 강도 테스트(Grip strength test)
    뇌 수분 함량 측정
    웨스턴 블롯 분석(Western blot analysis)
    면역형광 염색(Immunofluorescence staing)
결 과
    MCAO 수술로 인한 뇌 손상 시 바이칼린 투여로 인한신경행동 장애 완화
    MCAO 수술로 인한 뇌 손상 시 바이칼린 투여로 인한뇌 부종의 완화
    MCAO 동물에서 바이칼린 투여로 인한 γ-enolase 발현변화
고 찰
감사의 글
REFERENCES

• Hyun-Kyoung Son(Department of Veterinary Anatomy and Histology, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University)
• Phil-Ok Koh(Department of Veterinary Anatomy and Histology, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University) Corresponding author