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Next-Generation Sequencing–Based Development of Single Nucleotide Polymorphism Barcodes for Identification of the Cultivar ‘Kowinearly’ in Italian Ryegrass (Lolium multiflorum Lam.) KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/445058
pp.206-216
Molecular markers have been widely applied in population genetics, diagnostic taxonomy, and genetic mapping, and they can also be used for classifying varieties of Italian ryegrass during field selection. In this study, genome-wide sequence information was generated for 10 Italian ryegrass cultivars (40 samples), including ‘Kowinearly’ (KW), using next-generation sequencing (NGS). Single nucleotide polymorphism (SNP) analysis revealed that only three SNP loci were sufficient to distinguish KW from the other cultivars. Furthermore, 21 alternative barcode sets, each consisting of three SNPs, were identified. These SNP barcode sets provide a reliable criterion for cultivar discrimination in Italian ryegrass and can contribute to the protection of domestic varieties and the advancement of the forage industry in Korea. More broadly, the development of distinguishing markers across Italian ryegrass cultivars will enhance genetic resource identification and support the breeding of high-quality new varieties.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Plant materials
2. DNA extraction and library preparation
3. Preprocessing of sequencing data and SNP detection
4. Selection of cultivar-specific SNP markers
Ⅲ. RESULTS AND DISCUSSION
1. Sequencing data generation and preprocessing
2. SNP discovery through bioinformatic analysis
3. Phylogenetic tree and PCA analysis
4. Development of SNP barcode sets for cultivardiscrimination
Ⅳ. CONCLUSIONS
Ⅴ. ACKNOWLEDGMENTS
Ⅵ. REFERENCES
