Biological Activities of Solvent Fractions Fractionated with Celtus Methanol Extract
The physiological activities of solvent fractions derived from the celtus methanol extract were measured. Following the extraction of celtus with methanol, the yields of the resulting fractions—methanol, hexane, chloroform, ethyl acetate, butanol, and water—were found to be 19.57%, 3.24%, 26.02%, 8.15%, 8.72%, and 63.57%, respectively. The total flavonoid contents of the celtus methanol extract were 13.76, 4.96, 8.20, 31.99, 28.33, and 5.02 mg CE/g, respectively. The DPPH radical scavenging activity (IC50%) was measured at 22.48, 6.14, 11.15, 24.20, 19.18, and 20.71 μg/mL, with the highest activity observed in the ethyl acetate layer. The hydroxyl radical scavenging activity (IC50%) results were 14.27, 2.97, 9.19, 13.93, 3.90, and 3.10 μg/mL, also showing the highest activity in the ethyl acetate layer. The SOD-like activity measurements for the solvent fractions yielded values of 22.07, 9.65, 16.24, 32.63, 39.64, and 32.76 units/mL, with the highest activity in the butanol layer. The α-glucosidase inhibitory activity results were 16.42%, 11.29%, 16.60%, 27.40%, 2.86%, and 36.19%. Correlation analysis of antioxidant components and antioxidant activity indicated a very high correlation between total flavonoid content and both DPPH and SOD-like activities, suggesting that the celtus methanol extract positively influences antioxidant activity.