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궁채 메탄올 추출물로 분획된 용매분획물 생리활성 KCI 등재

Biological Activities of Solvent Fractions Fractionated with Celtus Methanol Extract

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

The physiological activities of solvent fractions derived from the celtus methanol extract were measured. Following the extraction of celtus with methanol, the yields of the resulting fractions—methanol, hexane, chloroform, ethyl acetate, butanol, and water—were found to be 19.57%, 3.24%, 26.02%, 8.15%, 8.72%, and 63.57%, respectively. The total flavonoid contents of the celtus methanol extract were 13.76, 4.96, 8.20, 31.99, 28.33, and 5.02 mg CE/g, respectively. The DPPH radical scavenging activity (IC50%) was measured at 22.48, 6.14, 11.15, 24.20, 19.18, and 20.71 μg/mL, with the highest activity observed in the ethyl acetate layer. The hydroxyl radical scavenging activity (IC50%) results were 14.27, 2.97, 9.19, 13.93, 3.90, and 3.10 μg/mL, also showing the highest activity in the ethyl acetate layer. The SOD-like activity measurements for the solvent fractions yielded values of 22.07, 9.65, 16.24, 32.63, 39.64, and 32.76 units/mL, with the highest activity in the butanol layer. The α-glucosidase inhibitory activity results were 16.42%, 11.29%, 16.60%, 27.40%, 2.86%, and 36.19%. Correlation analysis of antioxidant components and antioxidant activity indicated a very high correlation between total flavonoid content and both DPPH and SOD-like activities, suggesting that the celtus methanol extract positively influences antioxidant activity.

목차
Abstract
서 론
재료 및 방법
    1. 메탄올 추출물 및 용매분획물 제조
    2. 총 플라보노이드 함량 측정
    3. DPPH(1,1-diphenyl-2-picrylhydrazyl) 라디칼 소거능
    4. Hydroxyl 라디칼 소거능
    5. Superoxide dismutase(SOD) 유사활성
    6. α-Glucosidase 저해활성 측정
    7. 통계처리
결과 및 고찰
    1. 궁채 메탄올 추출물로 분획된 용매분획물의 플라보노이드 함량
    2. 궁채 메탄올 추출물로 분획된 용매분획물의 라디칼 소거능 측정
    3. 궁채 메탄올 추출물로 분획된 용매분획물의 superoxidedismutase(SOD) 유사활성
    4. 궁채 메탄올 추출물로 분획된 용매분획물의 α-glucosidase억제 활성 측정
    5. 궁채 메탄올 추출물로 분획된 용매분획물의 생리활성간의 상관관계
요약 및 결론
References
저자
  • 이연리(대전보건대학교 식품영양과 부교수) | Youn Ri Lee (Associate Professor, Dept. of Food and Nutrition, Daejeon Health Institude of Technology, Daejeon 34504, Korea) Corresponding author