Fenbendazole (FBZ) is one of benzimidazole drugs, which is well known for its broad spectrum of anthelmintics. During recent research on FBZ, it is also expected to have anti-inflammatory characteristics. However, the related research on FBZ with its anti-inflammatory effects is still lacking. This study focuses on the effects of FBZ on macrophage cell line of mouse, RAW264.7, and also on the inflammatory condition induced by lipopolysaccharide (LPS). FBZ alone treatment on RAW264.7 cells reduced the metabolic activity on the range of 1-5 μM, but the metabolic activity in the presence of LPS was higher than that of absence of LPS. Given that LPS has the ability to enhance glucose uptake in macrophage and rapid ATP production, there is a possibility that LPS increases the metabolic activity. Whereas TNF-alpha was hardly produced at FBZ 1-5 μM in the presence of LPS, IL-10 was still being produced, suggesting on the possibility of anti-inflammatory effect. Interestingly, despite the decreased survival of cells treated with FBZ, MHC class II and CD86 expression in surviving cells was markedly increased. Further investigation of FBZ is required through in vivo experiments to validate these findings.

Abstract
서 론
재료 및 방법
    세포주와 시약
    세포배양과 물질처리
    RAW264.7 세포의 대사활성도 측정
    사이토카인 생산량 측정
    유세포 분석
    세포사 측정
    통계분석
결 과
    RAW264.7 세포에서 FBZ를 단독 처리한 군과 LPS추가한 군의 대사활성도 감소
    FBZ와 LPS에 의한 사이토카인 형성
    FBZ와 LPS에 의한 면역세포 활성화마커 발현
    FBZ와 LPS에 의한 세포사 증가
고 찰
감사의 글
REFERENCES

• Dong-Ju Lee(College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea)
• Hong-Gu Joo(College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea, Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea) Corresponding author