This study quantitatively analyzed the target strength (TS) characteristics of the dotted gizzard shad (Konosirus punctatus) across various fork lengths (FL) and frequency conditions. In July 2023, TS measurements were conducted on six size groups (FL: 14.4–23.5 cm) under free-swimming conditions in a seawater acoustic tank at the Fisheries Resources Research Center in Tongyeong, Korea. A scientific echosounder (EK80, SIMRAD) was used to collect TS data at three frequencies: 38, 70, and 120 kHz. The results showed that TS values increased with fork length, and the 120 kHz frequency exhibited the widest distribution range and distinct bi- or multi-modal patterns. The TS–FL relationships for each frequency were as follows: TS38 kHz = 20·log(FL) ‒ 68.41, TS70 kHz = 20·log(FL) ‒ 70.76, and TS120 kHz = 20·log(FL) ‒ 70.90. Unlike traditional tethered measurement methods, this study obtained TS data under free-swimming conditions, providing values more representative of real-world acoustic survey environments. The findings are expected to serve as foundational data for improving the accuracy of monitoring the distribution and biomass estimation of K. punctatus using hydroacoustic methods.