Current assessments of stream ecosystem health in Korea using benthic diatoms rely primarily on the Trophic Diatom Index (TDI), which is highly sensitive to phosphorus concentrations but has limited ability to capture complex environmental stressors such as organic pollution and physical habitat degradation. To address these limitations and enhance the ecological diagnostic capacity of diatom-based assessments, we developed a Korean-type multimetric diatom index, the Diatom Assessment Index (DAI). Using benthic diatom assemblage data and environmental variables collected from 3,029 sites nationwide between 2019 and 2021, we screened candidate metrics based on variability, redundancy, discriminatory power, and sensitivity analyses. Five metrics-TDI, proportion of motile diatoms, proportion of sensitive diatoms, proportion of saprophilous diatoms, and the [Achnanthes / (Achnanthes+Navicula)] ratio-were ultimately selected and integrated to calculate the DAI score. Applying the DAI to an independent dataset from 3,005 sites (2022~2024) demonstrated that the index exhibited a near-normal distribution across assessment classes, in contrast to the TDI, which tended to be skewed toward specific ranges. The DAI showed strong correlations not only with physicochemical parameters such as BOD and TP but also with physical habitat indicators, including flow velocity and the proportion of fine substrates. In addition, the DAI was significantly correlated with other biological indices, such as the Benthic Macroinvertebrate Index (BMI) and the Fish Assessment Index (FAI). These results indicate that the DAI provides a more comprehensive and ecologically meaningful measure of stream health in Korea and can serve as an effective tool for national aquatic ecosystem assessment and management.