Biological assessments of streams have been developed in many countries to evaluate ecological integrity. A multimetric index is one of the primary methods used for this purpose, incorporating chemical, physical, and biological variables of the environment. In Korea, the Benthic Macroinvertebrates Index (BMI) is currently applied in national biological monitoring programs; however, BMI reflects only organic pollution and does not account for other environmental variables in streams. This study aimed to develop a new multimetric index, the Benthic Macroinvertebrate-based Multimetric Index (BMMI), for assessing the ecological integrity of Korean streams. We analyzed data from 3,307 sites, including water quality information. Reference and disturbed streams were identified based on PC 1 scores with 7 environmental factors (Axis 1 of the PCA), genus levelbased taxa richness, and BMGI values used for trimming. From an initial set of 82 candidate metrics, six (genus level-based taxa richness, Shannon’s diversity index, percent of taxa in E.P.T., percent of individuals in collectorsgatherers, percent of individuals in clingers, BMGI based on saprobity) were selected through statistical analyses, including coefficient of variation and discriminant analysis. BMMI successfully distinguished between reference and disturbed streams and showed significant correlations with various environmental factors. These results indicate that BMMI is suitable for evaluating the ecological integrity of streams in Korea. Therefore, it is recommended that stream ecosystem assessments transition from BMI to BMMI in the future to provide a more comprehensive evaluation of stream integrity.