More than half of the deaths caused by road accidents occur at night. For decades, engineers and scientists have been investigating and proposing numerous devices and measures to improve safety during nighttime driving. However, relatively new safety devices and applications are few, and their effects on nighttime driving remain ambiguous. Thus, the effects of a light-emitting diode (LED) road stud on night-time driving are investigated in this study. Thirty participants completed nighttime driving under four conditions on the same unlit mountainous road using a driving simulator. The four conditions were as follows: 1) no Rain/no studs, 2) rain/no studs, 3) rain/studs only on the centerline, and 4) rain/studs on the centerline and edges. Significant outcomes were obtained when rainy conditions were compared with clear weather conditions. The results suggest relative validity between the real world and a specified driving simulation. Significant safety benefits were indicated when comparing stud conditions (studs only on the centerline and studs on both sides) with the no-stud condition. Interesting results were obtained when comparing the case of studs only on the centerline with the case of studs on the centerline and on the edges. The right-edge crossing condition with LED studs on both sides showed better results by approximately 30% compared with the condition with LED studs only on the centerline. By contrast, the center-crossing condition with LED studs only on the centerline showed better results by more than 50% compared with the condition with LED studs on both sides.