This study presents the results of a comprehensive investigation of the macrofungal diversity in South Korea conducted between 2022 and 2025. Our approach has integrated detailed morphological observations with molecular analyses of large subunit (LSU) sequences. In the present study, we have identified four species, Retiboletus fuscus, R. sinensis, R. sinogriseus, and R. zhangfeii —that were previously unrecorded in South Korea. These findings significantly enhance the current understanding of fungal biodiversity within this region and also provide essential foundational data for advancing local and international mycological research.

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
    Sampling and morphological examination
    DNA extraction, polymerase chain reaction (PCR), andphylogenetic analyses
RESULTS
    Phylogenetic analysis
    Taxonomy
CONCLUSION
CONFLICTS OF INTERESTS
ACKNOWLEGEMENTS
REFERENCES

• Sung-Eun Cho(Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea)
• Jae Young Park(Q-myco Co, Seoul 08793, Republic of Korea)
• Jong Won Jo(Division of Microbiology, Honam National Institute of Biological Resources, Mokpo 58762, Republic of Korea)
• Nam Kyu Kim(Department of Forest Healing, College of Human service, Catholic Kwandong University, Gangneung 25601, Republic of Korea) Corresponding author