Alternative substrate materials for mushroom cultivation must be developed due to the unstable supply of the currently used substrates. Threonine acts as a nitrogen source in the substrate used for cultivating mushrooms. Threonine influences food flavor and participates in lipid metabolism and protein synthesis as well as energy metabolism through the tricarboxylic acid cycle. In this study, mushroom cultivation substrate was supplemented with threonine to evaluate the effects of threonine on the growth characteristics of and secondary metabolites produced by Pleurotus ostreatus. Threonine supplementation induced substantial changes in the parameters indicating the quality of fruiting bodies. These findings provide a basis for further research on supplementing substrates with amino acids for cultivating mushrooms.

ABSTRACT
서 론
재료 및 방법
    균주 및 접종원
    톱밥배지 내 균사 배양 및 자실체 생육 특성
    Threonine 처리에 따른 갓의 색차 조사
    Threonine처리에 따른 맛 조사
    Threonine 처리에 따른 향 조사
    통계분석
결과 및 고찰
    Threonine 첨가 배지 내 균사체 특성
    Threonine 첨가 배지 내 자실체 특성
    Threonine 처리에 따른 갓의 색차 조사
    Threonine 처리에 따른 맛 조사
    Threonine 처리에 따른 향 조사
적 요
감사의 말씀
REFERENCES

• 홍의용(공주대학교식물자원학과) | Eui-Yong Hong (Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea)
• 이진우(공주대학교식물자원학과) | Jin-Woo Lee (Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea)
• 송준형(공주대학교식물자원학과) | Jun-Hyung Song (Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea)
• 장명준(공주대학교식물자원학과, 스마트미래교육센터) | Myoung-Jun Jang (Department of Plant Resources, Kongju National University, Yesan 32439, Republic of Korea, Center for Smart Future Agricultural Education and Research, College of Industrial Sciences) Corresponding author
• 박윤진(두과녹비자원연구센터) | Youn-Jin Park (Kongju National University Legumes Green Manure Resource Center, Yesan 32439, Republic of Korea)