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시설재배 표고 참나무 톱밥 대체 배지자원 선발 KCI 등재

Selection of alternative substrate materials for oak sawdust facility cultivation of shiitake (Lentinula edodes)

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한국버섯학회지 (Journal of Mushrooms (J. Mushrooms))
한국버섯학회 (The Korean Society of Mushroom Science)
초록

This study aimed to identify and evaluate potential alternative substrate materials for the cultivation of Lentinula edodes (shiitake mushrooms) to address the growing instability in the supply of oak sawdust. A selection of locally abundant resources, including citrus branches, buckwheat hulls, Cryptomeria japonica sawdust, Miscanthus, and Sasa quelpaertensis, was evaluated as potential substitutes for oak sawdust. The results indicated that Miscanthus is rich in iron and manganese, which are essential cofactors for lignocellulose-degrading enzymes, such as cellulases, laccases, and peroxidases. In vitro mycelial growth assays (column tests) indicated that Miscanthus facilitated the most rapid growth among the alternative substrates for the shiitake strains ‘Chamaram’, ‘L808, ’ and ‘Haleumang’.’ For the strains 'Sanjo 701' and 'Nongjingo’, both oak sawdust and Miscanthus demonstrated comparable mycelial growth rates. Further cultivation tests with the strain ‘L808’ revealed that Miscanthus, whether used independently or in combination with oak sawdust, resulted in cultivation periods, primordia formation, and yield characteristics comparable to those achieved with the oak sawdust medium. These findings suggest that Miscanthus has significant potential as an alternative substrate for shiitake cultivation. However, as the study employed small, laboratory-scale blocks weighing approximately 300 g, while farm-scale blocks typically weigh 1.3–1.5 kg, fruiting was limited to a first flush harvest. Consequently, additional validation using farm-scale blocks is necessary to assess their practical applicability.

목차
ABSTRACT
서 론
재료 및 방법
    실험재료
    표고 균주 및 배지 제조
    버섯 발생 및 생육관리
    통계 분석
연구결과 및 고찰
    참나무 톱밥 대체재 자원 특성 조사
    대체재 종류에 따른 배지 특성
    대체재 종류에 따른 표고 품종별 균사 생장 속도
    억새 기반 배지에서의 배양 및 자실체 발생 특성
    배양기간 중 중량 감모율 변화
적 요
REFERENCES
저자
  • 이린아(제주특별자치도농업기술원) | Rina Lee (Agricultural Researcher, Jeju Special Self-Governing Province Agricultural Research and Extension Services, Seogwipo 63556, ,Republic of Korea) Corresponding author
  • 이정배(제주특별자치도농업기술원) | Jeongbae Lee (Agricultural Researcher, Jeju Special Self-Governing Province Agricultural Research and Extension Services, Seogwipo 63556, ,Republic of Korea)
  • 이강해(제주특별자치도농업기술원) | Kanghae Lee (Agricultural Researcher, Jeju Special Self-Governing Province Agricultural Research and Extension Services, Seogwipo 63556, ,Republic of Korea)
  • 김태균(제주특별자치도농업기술원) | Taegyun Kim (Agricultural Researcher, Jeju Special Self-Governing Province Agricultural Research and Extension Services, Seogwipo 63556, ,Republic of Korea)