Tricholoma matsutake, one of the most favorable ectomycorrhizal mushrooms, forms a symbiotic relationship with its host, Pinus densiflora. In this study, co-cultivation of T. matsutake mycelium with bacteria from fairy rings was conducted to explore artificial cultivation. Among the isolates tested, strain B22_9_B28 promoted mycelial growth across five T. matsutake isolates: KMCC05631 from Yangyang-gun (124.23%), KMCC05632 and KMCC05633 from Yeongdeok-gun (127.68% and 156.61%, respectively), and KMCC05634 from Yeongdong-gun (153.31%), and KMCC05644 from Bonghwa-gun (171.59%). 16S rRNA sequence analysis suggested that B22_9_B28 may represent a novel species of Paenibacillus. These results highlight the potential of a new Paenibacillus species with pan-regional growth-promoting effects on T. matsutake mycelium, offering a promising avenue for the artificial cultivation of this valuable mushroom.

• 최두호(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Doo-Ho Choi (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea)
• 임지훈(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Ji-Hoon Im (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea)
• 오연이(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Youn-Lee Oh (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea)
• 오민지(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | MinJi Oh (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea)
• 우성이(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Sung-I Woo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea)
• 이은지(농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) | Eun-Ji Lee (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong-gun, Chungbuk-do, 27709, Republic of Korea) Corresponding author