Melanoplus differentialis (Thomas, 1865) is a major pest of agricultural crops. In this study, we sequenced and characterized the complete mitochondrial genome of M. differentialis, which is 15,627 bp in length. The genome contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and an A+T-rich control region, with an overall nucleotide composition of 42.3% A, 32.5% T, 10.5% G, and 14.7% C. Phylogenetic analyses were performed using 13 PCGs and 2 rRNAs from available mitochondrial genomes of the family Acrididae. The results placed M. differentialis within the subfamily Melanoplinae, consistent with its current taxonomic classification. This newly sequenced mitochondrial genome provides a valuable resource for future studies on the evolution, phylogeny, and management of M. differentialis, enhancing our understanding of the genetic basis of this economically important pest.

Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgment
References

• Sanghyo Park(The Doctor’s Course Student, Department of Plant Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Seok Young Son(Researcher, Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon-si, 39660, Korea)
• Na Ra Jeong(The Doctor’s Course Student, Department of Plant Medicine, Gyeongsang National University, Jinju 52828, Korea)
• Jun-Ran Kim(Agricultural Researcher, Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon-si, 39660, Korea) Corresponding author
• Wonhoon Lee(Associate Professor, Department of Plant Medicine, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Korea) Corresponding author