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pp.388-396
This study evaluated the antioxidant and anti-inflammatory effects of the hot-water extract of Allium wakegi Araki roots (ARE). The extract was prepared by reflux extraction, concentration, and freeze-drying. Total polyphenol content (TPC) was measured using the Folin–Ciocalteu method and antioxidant activity was assessed by the DPPH radical scavenging assay. ARE showed dosedependent scavenging activity reaching 62.26±0.95% at 20 mg/mL. RAW 264.7 macrophages were used to examine anti-inflammatory activity. ARE did not exhibit cytotoxicity at concentrations ≤100 μg/mL. Lipopolysaccharide (LPS)-induced nitric oxide (NO) production was significantly reduced by ARE in a dose-dependent manner with the highest inhibition observed at 100, 200 μg/mL. qRT-PCR analysis revealed that ARE suppressed the mRNA expression of iNOS (Nos2) and COX-2 (Ptgs2). Western blot analysis further showed that ARE inhibited LPS-induced p-ERK phosphorylation and reduced the protein levels of iNOS and TNF-α. These results indicate that ARE possesses antioxidant capacity and effectively attenuates inflammation by inhibiting NO production, suppressing pro-inflammatory gene expression, and modulating MAPK signaling. Therefore, ARE may serve as a promising natural ingredient for functional foods targeting oxidative stress and inflammation.
서 론
재료 및 방법
1. 시료추출 및 고형분 함량 측정
2. DPPH 라디칼 소거활성
3. 총 폴리페놀 함량 측정(total polyphenol content, TPC)
4. 세포배양
5. 세포생존율 측정
6. NO 생성 억제량 측정
7. Quantitative real-time PCR(qRT-PCR) 분석
8. Western blot analysis
9. 통계처리
결과 및 고찰
1. 쪽파 뿌리 추출물의 고형분 함량 측정 결과
2. DPPH radical 소거능 측정 결과
3. 총폴리페놀 함량 측정결과
4. RAW264.7 세포의 생존율에 미치는 영향
5. NO 생성량 측정결과
6. ARE의 iNOS(Nos2) 및 COX-2(Ptgs2) mRNA 발현 감소 효과
7. ARE가 LPS로 유도된 RAW 264.7 세포의 MAPK 인산화에 미치는 영향
요약 및 결론
감사의 글
References
