Tissue stem cells are used for the regenerative medicine. In previous study we observed hard tissue formation of human dental pulp-derived cells using alginate scaffold. In this study, we explore the ability to differentiate of the 13th passage cells with glycerol 2-phosphate disodium salt hydrate (β-GP) which accelerate calcification. Reverse transcriptase Polymerase Chain Reaction (RT-PCR), transplants using alginate scaffold and histological examination were performed. We observed the expression of DSPP mRNA on day 10 cultured cells with β-GP. In conclusion, the 13th passage cells still have an ability to differentiate into odontoblast-like cells and alginate supports the differentiation of cultured cells in the transplants.

• Yasutomo Iwai(Department of Oral Anatomy, Osaka Dental University)
• Fumiko Aikawa(Department of Oral Anatomy, Osaka Dental University) Corresponding author
• Michiko Nakatsuka(Department of Oral Anatomy, Osaka Dental University)
• Shunji Kumabe(Department of Oral Anatomy, Osaka Dental University)
• Seong-suk Jue(Department of Oral Anatomy, School of Dentistry, Kung Hee University)
• Hiroyuki Hayashi(Department of Endodontics, Osaka Dental University)
• Je-won Shin(Department of Oral Anatomy, School of Dentistry, Kung Hee University)