This study investigated the feasibility of hydroponic cutting and cultivation for vegetative propagation and functional root production of Persicaria chinensis. Hydroponic cutting was conducted using distilled water under deep water culture conditions, and rooting characteristics were evaluated over a 14-day period. Rooting initiated at basal nodes two days after cutting and reached 100% by day 6. Compared with soil propagation, hydroponic cutting resulted in earlier rooting and more vigorous root development. Low-nutrient conditions suppressed shoot growth while promoting root elongation, leading to significant increases in root length and number over time. For hydroponic cultivation, plants were grown for up to eight weeks, and root biomass and gallic acid content were analyzed at two-week intervals. Root fresh and dry weights increased steadily throughout the cultivation period, reaching 444.87 mg and 38.47 mg, respectively, by week 8. In contrast, gallic acid content was highest at week 2 (2.88 mg/g) and gradually decreased thereafter, stabilizing after week 6. This trend suggests that early-stage physiological activity or stress responses under nutrient-limited conditions may enhance phenolic compound accumulation. These results demonstrate that hydroponic propagation is an effective approach for rapid multiplication and root production of P. chinensis, and highlight the potential of hydroponic systems for sustainable production of plant materials. Further optimization of nutrient solutions and light conditions is required to improve both biomass yield and secondary metabolite accumulation.

Abstract
Introduction
Materials and Methods
    1. Plant materials
    2. Evaluation of propagation feasibility using hydroponiccutting
    3. Evaluation of production potential using hydroponiccultivation
    4. Statistical analysis
Results and Discussion
    1. Evaluation of propagation feasibility using hydroponiccutting
    2. Evaluation of production potential using hydroponiccultivation
References

• Dae Ho Choi(Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea)
• Jung Mok Kang(Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea)
• Yong Woo Park(Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea) Corresponding author