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NGS-Based Development of SNP Barcodes for Variety Identification of ‘Alfaone’ in Alfalfa KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/449473
pp.38-48
Molecular markers have been widely utilized in population genetics, diagnostic taxonomy, and genetic mapping, and can be applied to cultivar discrimination during field selection processes for alfalfa. In this study, whole-genome sequencing information was obtained for seven alfalfa lines and cultivars developed in Korea, including ‘Alfaone (MS001)’, using Next-Generation Sequencing (NGS). Single nucleotide polymorphism (SNP) analysis revealed that ‘Alfaone (MS001)’ could be distinguished from other lines and cultivars using six SNP loci. Specifically, only two SNP loci were sufficient to differentiate ‘Alfaone (MS001)’ from major lines and cultivars such as ‘MS002’ and ‘Alfaking (MSCB07)’. This set of SNP barcodes provides a reliable standard for alfalfa cultivar discrimination, contributing to domestic cultivar protection and the advancement of the Korea forage industry. Furthermore, the development of distinguishing markers across alfalfa cultivars will enhance genetic resource identification and support the breeding of high-quality new cultivars.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Plant materials
2. DNA extraction and sequencing data production
3. Sequencing data preprocessing and SNP analysis
4. Phylogenetic tree and principal component analysis(PCA)
Ⅲ. RESULTS AND DISCUSSION
1. Sequencing data production and preprocessing
2. Read mapping and SNP extraction
3. Extraction of marker candidates for cultivardiscrimination
Ⅳ. CONCLUSIONS
Ⅴ. ACKNOWLEDGEMENTS
Ⅵ. REFERENCES
