This study evaluated the virucidal efficacy of a Bacillus complex composed of Bacillus subtilis YBK-70, Bacillus atrophaeus YBK-W4, and Bacillus circulans YBK-04 (1:1:1, v/v/v) against porcine epidemic diarrhea virus (PEDV). Virucidal efficacy was assessed by measuring the infectivity of PEDV in Vero cells after exposure of the virus to the Bacillus complex culture medium (BCCM) (4×10⁶ colony-forming units (CFU)/mL). PEDV was exposed to the BCCM in the presence of either hard water (HW) or an organic matter suspension (OM). Under HW conditions, PEDV was inactivated by a 50-fold dilution of the BCCM. In the presence of OM, the disinfectant exhibited virucidal activity at a 10-fold dilution. Because BCCM possesses virucidal activity against PEDV, it may serve as an effective reagent for limiting the spread of animal viral diseases.

Abstract
서 론
재료 및 방법
    복합미생물 조성
    공시바이러스 및 배양
    복합미생물 희석
    복합미생물과 PEDV 반응
    중화반응 및 PEDV 감염력 측정
    소독제 유효용량 및 권장용량 설정
결 과
    복합미생물 희석액 노출 PEDV 역가
    복합미생물의 유효용량 및 권장용량
고 찰
REFERENCES

• Sung Bum Lee(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Ik Ho Choi(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Youn-Tae Chi(Yessbio Co. Ltd., Gwangju 61005, Korea)
• Hu-Jang Lee(College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea) Corresponding author