Lactobacillus rhamnosus(1.0×109 CFU/g)와 inulin을 혼합한 synbiotics를 첨가하였을 때 잉어의 성장 및 생리적 반응을 조사하였다. 실험 시설은 순환여과식 사육 시스템이며, 잉어의 전장은 14.6±0.1 cm, 체중은 35.5±0.6 g이었다. 실험군은 대조구(control), 0.5(0.5% synbiotics), 1.5(1.5% synbiotics), 2.5(2.5% synbiotics) 그룹으로 설정하였고, 하루 두 번(09:00, 18:00) 어체중의 5%를 공급하였다. 시료 채취 및 계측은 실험 0(initial), 28일, 56일째에 진행하였다. 실험 결과 생존율은 대조구에서 유의하게 낮은 결과를 나타냈고(P<0.05), 성장은전장, 체중, GRL, GRW, ADG 및 Condition factor에서 대조구가 높고 2.5 그룹이 낮은 유의적 결과를 나타냈다(P>0.05). 혈액 성상인 RBC 및 Ht 분석 결과 모든 실험구 간 유의한 차이를 보이지 않았고(P>0.05), Hb에서 56일째에 2.5 그룹에서 유의하게 높아진 결과로 나타났다. 생화학적 지표인 ALT, BUN, TP, TG 및 TCHO는 모든 실험구에서 유의한 차이를 보이지 않았으며(P>0.05), AST는 2.5 및 대조구가 유의하게 낮은 결과를 나타냈다(P<0.05). 또한 ALP는 1.5 그룹에서 유의하게 낮아졌다. GLU는 0.5, 1.5 및 2.5 그룹에서 유의하게 낮은 결과를 나타냈다. 장 근위부의 융모의 길이 및 goblet cell의 수는 모든 실험구에서 차이가 없었다. 잉어에서 과도한 신바이오틱스 첨가는 성장의 둔화를 보이며, 혈액 성상과 BUN, TP, TCHO, TG, 및 조직학적 지표에 유의한 영양을 미치지 않는다고 판단된다. GLU는 대조구에서 가장 높게 나타나 신바이오틱스는 어느정도 면역 반응에 이점을 주는 것으로 판단된다.

This study investigated the effects of dietary synbiotics composed of Lactobacillus rhamnosus(1.0×10⁹ CFU/g) and inulin on the growth performance and physiological responses of common carp (Cyprinus carpio). The initial body weight is 35.5 ± 0.6 g, and the initial total length was 14.6 ± 0.1 cm. The fish were cultured in a recirculating aquaculture system and randomly assigned to four dietary treatment groups: control (0%), 0.5%, 1.5%, and 2.5% synbiotics. The fish were fed twice daily (09:00 and 18:00) at 5% of their body weight. Sampling and measurements were conducted at 0 (initial), 28, and 56 days. The survival rate was significantly lower in the control group (P<0.05). Growth parameters, including growth rate for body length, body weight, growth rate for total length(GRL), growth rate for body weight(GRW), average daily gain(ADG), and condition factor, were higher in control group, lower in the 2.5 groups, although no significant differences were observed among the treatments (P>0.05). Hematological parameters (RBC and Ht) showed no significant differences among all treatments (P>0.05). However, 56 day, a significant increase in Hb was observed in the 2.5 group. Similarly, biochemical indices (alanine aminotransferase (ALT), blood urea nitrogen (BUN), total protein (TP), triglyceride (TG), and total cholesterol (TCHO)) were not significantly affected (P>0.05). However, aspartate aminotransferase (AST) was significantly lower in the 2.5 and control groups (P<0.05), and ALP showed a significant decrease in the 1.5 group. GLU levels were significantly lower in the 0.5, 1.5 and 2.5 groups compared with the control (P<0.05). Histological observations of the intestine revealed no noticeable differences among treatments. Villus length and goblet cell counts in the proximal intestine is showed no significant differences among groups. These results suggest that excessive supplementation of synbiotics may suppress the growth of common carp, without significantly affecting hematological, biochemical (BUN, TP, TCHO, and TG), or histological parameters. The higher glucose (GLU) level observed in the control group indicates that synbiotic supplementation may confer some immunological benefits to the fish.

서 론
재료 및 방법
    1. 실험 어류 및 조건
    2. 성장 및 생존율
    3. 채혈 및 혈액분석
    4. 장의 조직학적 관찰
    5. 통계분석
결 과
    1. 생존율 및 성장
    2. 혈액성상
    3. 혈장의 GLU, ALP, AST, ALT, TP, TG, ALB및 TCHO
    4. 장의 조직학적 관찰
고 찰
사 사
참고문헌

• 문승일(국립군산대학교 해양생명과학과) | Seung Il Mun (Department of Aquaculture and Aquatic Science, Kunsan National University, Gunsan 54150, Republic of Korea)
• 윤형준(국립군산대학교 해양생명과학과) | Hyung Jun Yoon (Department of Aquaculture and Aquatic Science, Kunsan National University, Gunsan 54150, Republic of Korea)
• 허준욱(국립군산대학교 해양생명과학과) | Jun Wook Hur* (Department of Aquaculture and Aquatic Science, Kunsan National University, Gunsan 54150, Republic of Korea) Corresponding author