Polysaccharides possess various biological activities that can be applied in food, pharmaceutical, cosmeceutical, textile and paper industries. Although industrial polysaccharides are mainly produced by higher plant and seaweed, microorganisms are recognized as potential substitutes for the current major resources of polysaccharides due to their easy purification process and high production efficiency. In this study, we explored extracellular polysaccharides (EPS) produced by the fungus Gibellulopsis serrae strain JJ-W1 isolated from seawater. When cultured in media supplemented with three different carbon sources including glucose, maltose, and sucrose, the highest EPS production was observed in the glucose medium. In addition, the G. serrae EPSs showed inhibitory effects on collagenase activity. At a concentration of 20 mg/mL, collagenase activity was completely inhibited by the G. serrae EPSs, and the half-maximal inhibitory concentration(IC50) value was 6.21 ± 0.26 mg/mL. Based on the structural analysis of FT-IR spectra, the G. serrae EPSs exhibited different properties depending on the carbon sources. The EPSs produced in the glucose medium contained both α- and β-glycosidic linkages, whereas those from maltose or sucrose media contain only α-glycosidic bonds. Given the limited research on fungal polysaccharides and their collagenase inhibition, this study may provide basic information for their potential use in cosmetics.

Materials and Methods
    1. Sample collection and fungal isolation
    2. DNA extraction, polymerase chainreaction (PCR), and phylogenetic analysis
    3. Production of EPS in JJ-W1
    4. Fourier-transform infrared (FT-IR)spectroscopy
    5. Collagenase inhibition Assay
    6. Statistical analysis
Results and Discussion
    1. Identification of JJ-W1
    2. The effect of carbon sources onproduction of EPSs in G. serrae JJ-W1
    3. Inhibition of collagenase by G. serrae EPS
    4. Fourier-transform infrared spectroscopy(FT-IR)
Conclusion
Acknowledgements
References

• Woon-Jong Yu(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Ha Young Lee(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Haeun Bai(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Yong Min Kwon(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Seung Seob Bae(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Hyeon Gyeong Jeong(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea)
• Dawoon Chung(Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea) Corresponding author