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각질형성세포에서 블루베리 추출물의 항염증 기전 규명 연구 KCI 등재
Study on the anti-inflammatory mechanism of blueberry extract in HaCa T cells
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/450950
pp.160-172
Superfoods are rich in nutrients such as antioxidants, vitamins, and minerals. Blueberry (BB), one of these superfoods, is known for its beneficial effects on eye health, anti-aging, brain function, and immune enhancement. This study aimed to elucidate the pharmacological mechanisms of BB on skin inflammation. We investigated the antioxidant activities of BB by measuring DPPH, ABTS+ radical, and superoxide anion scavenging activities. In addition, we examined the effects of BB on inflammatory cytokine production and the activation of nuclear factor-κB (NF-κB) and caspase-1 in HaCa T cells. The results showed that the DPPH, ABTS+ radical, and superoxide anion scavenging activities of BB were 65.2%, 54.4%, and 58.3%, respectively. The total polyphenol and total flavonoid contents of BB were 44.1 mg GAE/g and 32.8 mg TA/g, respectively. BB significantly attenuated the production of inflammatory cytokines. Furthermore, we demonstrated that the anti-inflammatory effects of BB are mediated through the suppression of IκB-α degradation, NF-κB translocation, and caspase-1 activation in stimulated HaCa T cells. In conclusion, these findings suggest that BB may serve as a functional agent for mitigating skin inflammatory responses.
1. 서 론
2. 실 험
2.1. 재료
2.2. 시료 추출
2.3. 총 폴리페놀 함량 측정
2.4. 총 플라보노이드 함량 측정
2.5. DPPH radical 소거능 측정
2.6. ABTS radical 소거능 측정
2.7. Superoxide anion radical 소거능 측정
2.8. 세포 배양
2.9. 세포 생존율 측정
2.10. Enzyme-linked immunosorbent(ELISA) assay
2.11. Western blot analysis
2.12. Casapse-1 활성 측정
2.13. 통계처리
3. 결과 및 고찰
3.1. 블루베리 추출물의 항산화 효능
3.2. 블루베리 추출물의 총 폴리페놀 및 플라보노이드 함량 측정
3.3. 블루베리 추출물의 세포독성 및 염증성사이토카인 조절 효과
3.4. 블루베리 추출물의 NF-kB 활성조절 효과
3.5. 블루베리 추출물의 Caspase-1 활성 조절효과
4. 결 론
감사의 글
References
