This study was conducted to evaluate the effects of cultivation periods and substitution levels of sprouted barley on chemical compositions, fermentation characteristics, microbial counts, and nutrient digestibility of fermented-total mixed ration (F-TMR). Sprouted barley (SB) was cultivated for 6 (SB6) and 8 days (SB8), respectively, using a hydroponic system. It was harvested and chopped for substitution at 0, 5, or 10% (as-fed basis) in experimental diets (TMRs). And, the TMRs were then ensiled into the bucket silo (20 L) for 7 days to produce F-TMR (n=3). As the sprouting period increased, crude protein (p=0.019; SB6 vs. SB8 = 18.7% vs. 19.1%) and non-fiber carbohydrate (p<0.001; 35.4% vs. 36.4%) increased, whereas the crude ash (p=0.003; SB6 vs. SB8 = 7.15% vs. 6.95%) decreased. These differences were maintained in F-TMR (p<0.05). Lactate (p=0.013; 5% vs. 10% = 2.49% vs. 2.76%) and propionate (p<0.001; 0.31% vs. 0.49%) contents in F-TMR increased by increasing the substitution level of SB, but pH (p<0.001; 5.20 vs. 4.98) decreased (p<0.05). Lactic acid bacteria (p=0.017; 5% vs. 10% = 7.37 log10 cfu/g vs. 7.71 log10 cfu/g) and Bacillus subtilis (p=0.002; SB8-5% vs. SB8-10% = 6.90 log10 cfu/g vs. 7.49 log10 cfu/g) of F-TMR increased by increasing the substitution level of SB. Furthermore, in vitro digestibilities of dry matter (p<0.001; 5% vs. 10% = 72.8% vs.74.8%) and neutral detergent fiber (p<0.001; 48.8% vs. 55.0%) also increased by increasing the substitution level of SB. Therefore, this study suggested that SB cultivated for 8 days could be used as a feed source in TMR to improve fermentation quality and nutrient utilization of F-TMR, particularly at a 10% substitution with grain mixture.

ABSTRACT
Ⅰ. 서론
Ⅱ. 재료 및 방법
    1. 보리 생산 및 TMR 제조
    2. 영양소 함량, 발효특성 및 미생물 성상
    3. 영양소 소화율
    4. 통계처리
Ⅲ. 결과 및 고찰
    1. 시험사료의 발효 전 영양소 함량
    2. 시험사료의 발효 후 영양소 함량과 발효특성
    3. 시험사료의 발효 후 미생물 성상 및 소화율
Ⅳ. 요약
Ⅴ. 사사
Ⅵ. REFERENCES

• 김지윤(경상국립대학교 응용생명과학부(BK21 Four)) | Ji Yoon Kim (Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea)
• 백창현(경상국립대학교 응용생명과학부(BK21 Four)) | Chang Hyun Baeg (Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea)
• 최부길(경상국립대학교 응용생명과학부(BK21 Four)) | Bu Gil Choi (Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea)
• 김삼철(경상국립대학교 응용생명과학부(BK21 Four), 경상국립대학교 축산과학부(농업생명과학연구원)) | Sam Churl Kim (Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of Korea, Division of Animal Science (Institute of Agriculture and Life Science), Gyeongsang National University, Jinju 52828, Republic of Korea) Corresponding author
• 허태웅(경상국립대학교 축산과학부(농업생명과학연구원)) | Tae Wong Hur (Division of Animal Science (Institute of Agriculture and Life Science), Gyeongsang National University, Jinju 52828, Republic of Korea)
• 정승민(국립축산과학원 조사료생산시스템과) | Seung Min Jeong (Forages Production Systems Division, National Institute of Animal Science, RDA, Cheonan 31000, Republic of Korea)
• 주영호(경상북도 축산기술연구소) | Young Ho Joo (Gyeongsangbuk-do Livestock Research Institute, Yeongju 36052, Republic of Korea)