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Development of an F₂ Population and QTL Analysis for Resistance to Bacterial Wilt in Pepper (Capsicum annuum L.) Using Genotyping-by-Sequencing KCI 등재
GBS 기반 고추 풋마름병 저항성 QTL 분석 및 F₂ 집단 육성
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/451877
pp.151-158
Bacterial wilt, caused by Ralstonia solanacearum, is one of the most destructive soil-borne diseases a ffecting p epper ( Capsicum annuum L.) production. Developing resistant cultivars is considered the most effective strategy for sustainable disease management. This study investigated the genetic basis of bacterial wilt resistance using an F₂ population derived from a cross between the resistant line ‘FRH1’ a nd t he s uscep tible line ‘ Saengryeok211’. A total o f 180 F ₂ individuals were evaluated for disease response following artificial inoculation with R. solanacearum. Disease severity was assessed using a disease index (DI) scale based on wilting symptoms, and the distribution of DI values showed continuous variation. Classifying individuals with DI 0–1 as resistant and those with DI 2–4 as susceptible, the segregation ratio fit the expected 1:3 ratio, suggesting that resistance may involve a recessive major gene. A genetic linkage map was c onstructed u sing single nucleotide p olymorp hism ( SNP) m arkers d erived from genotyping-by-sequencing (GBS) data. Composite interval mapping subsequently identified a genomic region on chromosome 5 associated with bacterial wilt resistance. This identified Quantitative Trait L ocus ( QTL) e xp lained a s ignificant p rop ortion o f phenotyp ic v ariation a nd o ffers v aluable information for marker-assisted selection in pepper breeding programs.
INTRODUCTION
MATERIALS AND METHODS
Plant materials
Inoculation and Disease evaluation
Genetic linkage map construction
QTL Analysis
RESULTS
Phenotypic variation of bacterial wilt resistance
Segregation analysis of bacterial wilt resistance
Genetic linkage map
Identification of QTL for bacterial wilt resistance
Discussion
적 요
REFERENCES
