Selection of Abiotic Stress-Tolerant Rice Elite Lines and Analysis of Regional Adaptability through the AFACI Multinational Breeding Network
This study aimed to strengthen food security in 10 AFACI (Asian Food and Agriculture Cooperation Initiative) member countries by developing climate-resilient rice (Oryza sativa L.) lines and establishing a multinational breeding network. The initiative responds to the climate crisis by focusing on elite rice lines tolerant to abiotic stresses (drought, salinity, and submergence). An analysis of rice cultivation e nvironments in these c ountries r evealed that a p proximately 3 5.2% ( 16.74 M ha) of t he t otal area (47.6 Mha) is disaster-prone. Drought was the most prevalent threat (19.9%), followed by salinity (8.3%) and submergence (7.9%). Bangladesh (62.7%) and Thailand (60.6%) showed the highest sensitivity to these stresses. Using the Standard Evaluation System (SES) and an Augmented-RCBD design, 86 elite lines were selected through multi-location 'hotspot' testing. These included 32 lines for drought tolerance, 29 for salinity tolerance, and 25 for submergence tolerance. Notably, IR16F1148 (tolerant to salinity and submergence) and IR13084-B-1456-1 (tolerant to drought and submergence) were identified as key genetic resources for addressing climate variability. These lines will undergo further evaluation in Stage 2 Multi-environment Trials (MET) to facilitate rapid variety registration and dissemination. The established breeding network, supported by standardized digital data management via the Enterprise Breeding System (EBS), enhances breeding efficiency and ensures data integrity. This network serves as a critical foundation for climate-resilient agricultural productivity in the AFACI region.