This study aimed to investigate the electrophoretic patterns of serum proteins in clinically healthy dogs and in dogs suffering from acute pancreatitis or pyometra, using agarose gel electrophoresis. The primary objective was to examine how health status influences the distribution and concentration of serum protein fractions. Blood serum samples were collected from a total of 26 dogs, including 20 dogs diagnosed with acute pancreatitis or pyometra and 6 clinically healthy control dogs. Total serum protein concentrations were determined, and both absolute and relative values of individual protein fractions were analyzed. Serum protein electrophoresis revealed distinct patterns in both healthy dogs and those with systemic diseases. In dogs with acute pancreatitis or pyometra, total serum α2 protein concentrations were significantly higher, whereas albumin levels were significantly lower than in healthy dogs. However, globulin (g/dL) levels showed no significant difference between healthy dogs and those with acute pancreatitis, whereas dogs with pyometra showed significantly higher values. The results presented suggest a more pronounced influence of health on the concentrations of several serum protein fractions in dogs. Seeing that the analysis of serum proteins and their electrophoretic separation in systemic disease dogs is less well documented, the study provides important findings for clinicians when evaluating dysproteinemias.

Abstract
INTRODUCTION
MATERIALS AND METHODS
    Animals and sample collection
    Serum electrophoresis
    Data analysis
RESULTS
DISCUSSION
REFERENCES

• Ji-Yeong Ku(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Jang-Hwan Lim(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Khankham Bouphavanh(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Hyungjae So(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Lianfu Piao(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Dong-ho Noh(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Jonghyun Lee(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Yujin Ye(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea)
• Jinho Park(Department of Veterinary Internal Medicine, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea) Corresponding author