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Biological Activities of Spinacia oleracea L. Leaf and Stem Extracts and Their Potential Applications in Cosmetics KCI 등재
시금치 (Spinacia oleracea L.) 잎 및 줄기 추출물의 생리활성 비교 및 화장품 소재로서의 활용 가능성 평가
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/452928
pp.659-673
본 연구에서는 70% 에탄올로 추출한 시금치 (Spinacia oleracea L.) 잎 추출물(SOL)과 줄기 추출물(SOS)의 항산화 및 항염증 활성을 비교하고, 화장품 소재로서의 활용 가능성을 평가하였다. 항산 화 활성은 ABTS+ 및 DPPH 라디칼 소거능 분석을 통해 평가하였으며, 총 폴리페놀 및 총 플라보노이드 함량을 함께 분석하였다. 그 결과, SOL은 SOS보다 높은 라디칼 소거 활성과 폴리페놀 및 플라보노이드 함량을 나타냈다. 항균 활성은 Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), 및 Cutibacterium acnes (C. acnes)를 대상으로 평가하였으나, 두 시 료 모두 시험 균주에 대해 유의적인 항균 활성을 나타내지 않았다. 항염증 활성 평가 결과, SOL과 SOS 는 lipopolysaccharide (LPS)로 자극된 RAW 264.7 대식세포에서 nitric oxide (NO) 생성과 inducible nitric oxide synthase (iNOS) 및 cyclooxygenase-2 (COX-2) 단백질 발현을 농도 의존적으로 억제하 였으며, 특히 SOL에서 더욱 우수한 억제 효과가 확인되었다. 또한 형태학적 관찰 결과, SOL은 염증 자 극에 의해 유도된 세포 형태 변화를 완화하는 경향을 나타냈다. 화장품 적용 가능성을 평가하기 위해 SOL을 함유한 에멀젼 제형을 제조하고 안정성을 분석하였다. 그 결과, SOL 함유 에멀젼은 실온 및 고 온 저장 조건에서 안정적인 pH와 점도를 유지하였으며, 상분리 및 침전과 같은 물리적 불안정성도 관찰 되지 않았다. 따라서 본 연구 결과는 SOL이 우수한 항산화 및 항염증 활성을 기반으로 기능성 화장품 소재로 활용될 가능성이 있음을 시사한다.
This study compared the antioxidant and anti-inflammatory activities of spinach (Spinacia oleracea L.) leaf extract (SOL) and stem extract (SOS) prepared using 70% ethanol and evaluated their potential as cosmetic ingredients. Antioxidant activity was assessed using ABTS⁺ and DPPH radical scavenging assays, together with measurements of total polyphenol and flavonoid contents. SOL exhibited stronger radical scavenging activity and higher polyphenol and flavonoid contents than SOS. Antibacterial activities against Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Cutibacterium acnes (C. acnes) were evaluated; however, neither SOL nor SOS showed significant antibacterial effects against the tested bacterial strains. The anti-inflammatory activities of SOL and SOS were investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both extracts dose-dependently suppressed nitric oxide (NO) production and reduced the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), with SOL showing more pronounced inhibitory effects than SOS. In addition, microscopic observations revealed that SOL alleviated inflammation-induced morphological changes in RAW 264.7 cells. To evaluate cosmetic applicability, an emulsion containing SOL was formulated and subjected to stability testing. The SOL-containing formulation maintained stable pH and viscosity under room temperature and elevated temperature storage conditions without noticeable phase separation or precipitation. Taken together, these findings suggest that SOL possesses potent antioxidant and anti-inflammatory activities and may serve as a promising functional cosmetic ingredient.
요 약
1. Introduction
2. Materials and Methods
2.1. Preparation of SOL and SOS Extract
2.2. Reagents and Antibodies
2.3. ABTS+ Radical Scavenging Activity
2.4. DPPH Radical Scavenging Activity
2.5. Total Flavonoid Content
2.6. Total Polyphenol Content
2.7. Bacterial Strains and GrowthConditions
2.8. Disc Diffusion Assay
2.9. Cell Culture
2.10. Cell Viability
2.11. NO Production
2.12. Western Blot
2.13. Formulation of SOL-ContainingEmulsion
2.17. Stability of the Formulation
2.18. Statistical Analysis
3. Results and Discussion
3.1. Antioxidant effect of SOL and SOS
3.2. Total polyphenol and flavonoidcontents of SOL and SOS
3.3. Antibacterial activities of SOL andSOS against P. aeruginosa, S.aureus, E. coli, C. acnes.
3.4. Anti-inflammatory effects of SOL andSOS in LPS-stimulated RAW 264.7cells
3.5. Stability evaluation of SOL-containingemulsion formulation
4. Conclusion
Acknowledgement
References
