Background: Widespread smartphone utilization often leads to poor postural adaptations that alter musculoskeletal alignment, thereby adversely affecting muscle activity, respiratory function, and pain thresholds. Objectives: This study investigated the effects of desk-to-trunk distance on cervical muscle activity, respiratory function, and pressure pain threshold (PPT) during smartphone use in healthy young adults. Design: Randomized crossover trial. Methods: Twenty young adults who use smartphones for an average of four hours or more per day performed a 20-minute smartphone task under three distance conditions: 0, 15, and 30 cm. Muscle activity of the splenius capitis (SC) and upper trapezius (UT), respiratory function and PPT were assessed pre- and post-intervention. Results: Right UT muscle activity was significantly higher at 30 cm compared to 0 cm (P=.01). Significant decreases were observed in forced vital capacity (P=0.04) and slow vital capacity (P=.03) at 15 cm, and in maximum voluntary ventilation at 30 cm (P=.02) between the pre- and post-tests. The reduction in bilateral SC PPT was significantly more pronounced at 30 cm than at 0 cm (P=.01). Notably, significant PPT declines were observed across all conditions (right UT [P=.03], left SC [P=.01] at 0 cm; right UT [P=.03] at 15 cm; left SC, right SC, left UT, and right UT [P<.01] at 30 cm). Conclusion: Increased desk-to-trunk distance exacerbates forward head posture, reducing respiratory efficiency and increasing pain sensitivity, making minimal proximity (0 cm) an effective ergonomic strategy.