Background: The recent instability in youth employment and the high unemployment rate among youth contribute to the employment stress experienced by graduating university students. Objectives: The aim of this study is to examine the relationship between major selection motive, employment stress and employment preparation behavior of graduating physical therapy students. Design: Questionnaire design. Methods: A total of 171 graduating physical therapy students were surveyed to assess their motive for choosing a major, their employment stress, and their employment preparation behavior. Then, the correlations between these parameters were analyzed. Results: The results showed that students with high satisfaction with college life had significantly stronger motivation to choose their major, positive job preparation behaviors, and lower levels of job stress (P<.05). Moreover, high satisfaction with one’s major indicates a significantly strong motivation for major selection and positive employment preparation behavior (P<.05). A positive correlation was observed between motive for major selection and employment preparation behavior (P<.05). Conclusions: The motive for choosing a major and the employment preparation behavior of graduating physical therapy students must be examined in order to provide tailored support to prepare them for employment.