대두 저장 단백질의 glycinin(11S globulin)과 β-conglycinin(7S globulin)은 대두 가공식품의 품질을 결정짓는 핵심 인자이다. 본 총설에서는 초원심분리(Analytical Ultracentrifugation, AUC) 및 SDS-PAGE, Densitometery, Perfusion 역상 고성능 액체크로마토그래피(Perfusion Reversed-Phase High-Performance Liquid Chromatography, Perfusion RP-HPLC), 효소 면역 측정법(Enzyme-Linked Immunosorbent Assay, ELISA), Dumas method부터 최근 펩타이드 전용 컬럼을 활용하여 18분 이내에 10대 주요 서브유닛(11S: G1, G2, G3, G4, and G5; 7S: α’, α, and β)을 완전 분리·정량하는 역상 초고성능 액체크로마토그래피(Reversed-Phase Ultra-Performance Liquid Chromatography, RP-UPLC) 에 이르는 분석 기술의 발전 과정을 고찰하였다. 나아가 11S/7S 분획비 및 개별 서브유닛 조성과 두부, 두유, 그리고 식물성 대체육(Plant-Based Meats, PBMs)의 가공성과의 관계를 소개하여 정밀 서브유닛 프로파일링이 콩 가공식품 품질 균일화 및 개발을 위한 원료 선별 및 맞춤형 분자 육종의 자료로 유용하게 활용될 수 있음을 시사한다.

Glycinin (11S globulin) and β-conglycinin (7S globulin), the major storage proteins in soybeans, are key determinants of the quality of processed soy food products. This review examines the analytical evolution from conventional methods—such as analytical ultracentrifugation (AUC), SDS-PAGE, densitometry, perfusion reversed-phase high-performance liquid chromatography (perfusion RP-HPLC), enzyme-linked immunosorbent assay (ELISA), and the Dumas method to recent reversed-phase ultra-performance liquid chromatography (RP-UPLC) using a peptide-dedicated column, which achieves complete separation and quantification of the ten major subunits (11S: G1, G2, G3, G4, and G5; 7S: α’, α, and β) within 18 minutes. Furthermore, by elucidating the relationships between the 11S/7S fractionation ratio, individual subunit compositions, and the processing suitability of tofu, soymilk, and plant-based meats (PBMs), this review suggests that precise subunit profiling serves as a valuable tool for raw material selection and tailored molecular breeding to enhance the quality uniformity and development of processed soy foods.

초록
Abstract
서론
    1. 대두 저장단백질의 분석기술 및 연구 동향
    2. RP-UPLC를 이용한 11S, 7S Globulin 정성⋅정량 분석
    3. 두부(Tofu) 가공성과 glycinin 및 β-conglycinin의상관 관계
    4. 두유(soy milk) 가공성과 glycinin 및 β-conglycinin의상관 관계
    5. 식물성 대체육(PBMs) 가공성과 glycinin 및 β-conglycinin의상관 관계
결론 및 향후 연구 방향
감사의 글
References

• 이수빈(전북대학교 농업생명과학대학 식품공학과 박사과정, 농촌진흥청 국립농업과학원 농업생명자원부 디지털육종지원과 학연연구원) | Su Bin Lee (Ph.D. Student, Department of Food Science and Technology, Jeonbuk National University, Jeonju, 54896, Korea, Joint Ph.D. Student, Digital Breeding Convergence Division Department of Agricultural Biotechnology, Rural Development Administration, Jeonju, 54874, Korea)
• 하기훈(농촌진흥청 국립농업과학원 농업생물부 식물소재바이오공학과 농업연구사) | Kihun Ha (Agricultural Researcher, Plant Biomaterials and Biotechnology Division Department of Agricultural Biology, Rural Development Administration, Jeonju, 54874, Korea)
• 이재훈(전북대학고 농업생명과학대학 식품공학과 조교수) | Jae Hoon Lee (Assistant Professor, Department of Food Science and Technology, Jeonbuk National University, Jeonju, 54896, Korea)
• 김세원(농촌진흥청 국립농업과학원 농업생명자원부 디지털육종지원과 전문연구원) | Sewon Kim (Postdoctoral Researcher, Digital Breeding Convergence Division Department of Agricultural Biotechnology, Rural Development Administration, Jeonju, 54874, Korea) Corresponding author
• 이종열(농촌진흥청 국립농업과학원 농업생명자원부 디지털육종지원과 농업연구관) | Jong Yeol Lee (Senior Agricultural Research, Digital Breeding Convergence Division Department of Agricultural Biotechnology, Rural Development Administration, Jeonju, 54874, Korea) Corresponding author