Oral squamous carcinoma (OSC) is the most common malignant neoplasm of the oral mucosa. Although the etiology of OSC is not fully understood, accumulated evidences indicate that the activation of proto-oncogenes and the inactivation of tumor suppressor genes underlie the disease development. An OSC cell line, YD-9 was newly established and characterized. However, the mutational analysis of p53 gene was not performed. Thus, in this study, the presence of mutation in the p53 gene was examined by amplification of exon-4 to -8 and subsequent DNA sequencing. Two point mutations were found in exon-4 and -6: A to G, resulting in amino acid change Tyr to Cys in exon-4, and C to G, resulting in amino acid change Gly to Arg in exon-6, respectively. Any mutation was not found in the exon-5, -7 and -8. The presented results would contribute to basic research to understand the biological mechanism of OSC using YD-9 cells.

• Gyoo-Cheon Kim(Department of Oral Anatomy, School of Dentistry, Pusan National University) Corresponding author
• Ji-Hak Min(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Do-Kyun Kim(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Moo-Hyung Lee(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Moon-Kyoung Bae(Department of Oral Physiology, School of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
• Kyung-Il Um(Department of Biology, College of Natural Science, Dong-A University)
• Hyun-Ho Kwak(Department of Oral Anatomy, School of Dentistry, Pusan National University)
• Bong-Soo Park(Department of Oral Anatomy, School of Dentistry, Pusan National University)