논문 상세보기

수정란의 급속동결융해법에 관한 연구. III. 소 동결수정란에 대한 1단계 Straw법이 난자 생존성에 미치는 영향 KCI 등재

Studies on Quick Freezing and Thawing of Embryos. III. Survival Effects of Bovine Embryos Cryopreserved and Diluted by One-Step Straw Method for Handling of Frozen-Thawed Embryos

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/47031
구독 기관 인증 시 무료 이용이 가능합니다. 4,300원
한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

The objective of this experiment was to study some possibilities to simplify freezing, thawing and transfer procedure of one-step straw method comparing with the conventional methods using bovine embryos. The previous work are also designed to investigate the thawing effect by development stage and its quality using the embryos. Results obtained were summarized as follows: 1. A total of 87 embryos from 14 donor cows were frozen-thawed and an average of frozen embryo/donor was 6.2. 2. The survival rates of morula stage(65.4%) were higher than those of blastocyst stage(57.l%) and vice versa in rate of morphological recovery (80% vs 95.4%). However. no significant difference was denoted between them. 3. In difference between the groups of good quality and poor quality. good quality was resulted in a significantly higher embryo survival rate(75%) and recovery rates(95%) than poor quality(P<0.0l). 4. In effects of non-permeable sugar dilution in added to l.0M glycerol. higher survival rates were orderd in sucrose. lactose, raffinose and xylose. But lactose-raffinose, sucrose-trehalose and xylose in added to 2.OM glycerol. 5. The highest survival rates were obtained by direct plunge into the liquid nitrogen with 3.OM concentration both of glycerol and trehalose. 6. The survival rates in vitro condition of one-step and direct plunge methods(75%-87.5%) were significantly higher than those of multiple steps (21.4-52.6%) in in vitro (P<0.0l). However, the results of single-step were critical in comparing to other steps of final pregnant conformation.

저자
  • 석호봉(단국대학교 농과대학)
  • 이광원(국립종축원)
  • 손동수(국립종축원)
  • 김일화(국립종축원)